- Conduct a comprehensive literature review of the likelihood of production of E. coli bacteria of freshwater fishes found in warm water streams;
- To determine whether wild caught fish from Harris County waterways representing various species and trophic groups produce feces with detectable levels of the indicator bacteria E. coli;
- Determine whether fish retained in aquaria transmit E. coli bacteria to ambient water;
- Conduct a literature review to determine likely densities of native fish in urban bayous and feces production rates; and
- Using these data, estimate potential loading rates of E. coli bacteria into urban bayous from freshwater fishes.
White Oak Bayou, Greens Bayou, Horsepen Bayou
2008 – 2009
The goal of this study was to determine whether wild caught fish from Harris County waterways representing various species and trophic groups produce feces with detectable levels of the indicator bacteria E. coli. Fish were collected during each sampling period using an electroshocking tote barge. Fish were then euthanized and fecal matter was obtained by dissecting the intestinal tract. The fecal material was then placed into a pre-weighed bottle of sterile water. Ambient water quality was measured during field collection of fish. All water and fish fecal suspension samples were analyzed using the IDEXX Collilert® defined substrate method which has been approved for use in Texas by both the TCEQ and EPA and is described in TCEQ (2003).
We also completed an aquarium E. coli production study to determine the potential for E. coli to originate within a fish. We used cultured fish which were fed prepared fish meal to determine whether E. coli levels increase in aquarium water after exposure to fish at various stocking levels. If E. coli originates from the fish itself, we would expect levels of bacteria in water to increase with fish density and time irrespective of origin of the fish. Two species (bluegill and channel catfish) of fish from different trophic levels were used for this portion of the study.
Publications and Presentations
Guillen, G. and Wrast, J. 2010. Fishes as sources of E. coli bacteria in warm water streams. EIH Technical Report 10-015:81.
Ramirez, D., Franks, M., Wrast, J., and Guillen, G. 2009. Fish as possible sources of indicator bacteria in urban streams. Texas Chapter of the American Fisheries Society Meeting, Fort Worth, Texas. Oral Presentation.